Cytokeratin 5 Antikörper (AA 318-491)

Produktnummer ABIN6934855

Dieses Anti-Cytokeratin 5-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cytokeratin 5 in IHC (f). Geeignet für Human.

Kurzübersicht für Cytokeratin 5 Antikörper (AA 318-491) (ABIN6934855)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 5 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: KRT5-2080

Produktdetails anti-Cytokeratin 5 Antikörper

Bindungsspezifität: AA 318-491

Spezifität: This MAb recognizes a protein of 58 kDa, which is identified as Cytokeratin 5 (KRT5). This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to KRT5 identify basal cells of squamous and glandular epithelia, myoepithelia, and mesothelium.Anti-cytokeratin 5 has been reported useful in the differential diagnosis of metastatic carcinoma in the pleura versus epithelioid mesothelioma. Almost all squamous cell carcinomas, half of transitional carcinomas, and many undifferentiated large cell carcinomas express.Anti-KRT5, along with anti-p63, affords a high sensitivity and specificity for squamous differentiation. Myoepithelial cells of the breast, glandular epithelia, and basal cells of the prostate are labeled with anti-KRT5.

Kreuzreaktivität (Details): Human.

Immunogen: Recombinant human Cytokeratin 5 (KRT5) protein fragment (around aa 318-491) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HeLa cells. Tonsil, Esophagus, Cervix or Urinary Bladder. Basal Cell or Squamous Cell Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Tissue culture supernatant with 0.05% Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Andere Bezeichnung: KRT5

Hintergrund: 58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 5 (KRT5) (Basal, Myoepithelial & Mesothelial Cell Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 58kDa

Gen-ID: 3852, 433845

UniProt: P13647